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ThermanoxTM盖玻片，已灭菌，外部尺寸，25直径
  • 商城价格 登录后可查看价格
  • 货号 174985
  • 品牌 Thermo Fisher/赛默飞世尔 ( 经销商 )
  • CAS号
  • 规格/包装 箱
  • 单位 箱
  • 储存条件 室温
  • 现货状态 一个月
  • 数量

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Thermanox® or TMX Coverslips，圆形细胞培养用塑片，方形细胞培养用塑片均来自NUNC。该培养片由特殊塑料（聚烯烃的聚合体）制造，能抵抗很多种化合物的浸蚀；对乙醇、乙醛、碳氢化合物、稀酸（<10%）、稀碱（<2%）均不反应；对于氯化碳氢化合物部分抵抗；对于浓酸和浓碱不抵抗。此塑料具有延展性、透明，用切片机可以切；耐高温(temperature range –70ºC to +150ºC)。塑片只有一面经过特殊处理（朝着商标的这面）。可以用灭菌剪刀裁出任意形状。用灭菌镊子夹取，注意刮擦，请拿取塑片边缘；可以用镊子弯曲塑片的一角形成拿取把手。

  • 平整的表面
  • 0.2mm厚
  • 与常用溶剂不反应
  • 可经受20分钟的120°C湿热或者干热灭菌
    玻片一侧经过处理，最适合细胞粘附和生长
  • 当使用解剖刀或切片机进行切割时，不易破裂
  • 辐射灭菌
  • 自身荧光范围 380 到 545 nm
  • 安全和易于处理

    • 应用：非常适用于电镜样本。激光共聚焦使用，请避开自发荧光范围380 到 545 nm。
    塑料细胞爬片有极大的优势，可以用灭菌的剪刀裁剪适合的大小方便不同的应用。可以用切片机切做免疫染色或是免疫荧光。
    应用实例：http://www.cjcb.org/news/upload/201612211603376545.pdf

  • Purpose

    Cell attachment and growth is equal to or better than polystyrene plastic or glass.

    • Resistant to all commonly used solvents (xylene, acetone, acetic acid) so you can use them with most staining techniques and with regular mounting and embedding materials
    • Use with amyl acetate for EM preparations.
    • Very low vapor/gas permeability properties.
    • Suitable for use in scintillation counting.

    Warning

    • Coverslips are packaged cell culture slide toward label. To assure best orientation for cell growth, keep coverslips in package until ready for use. When removing from package, Use side facing labeled top of package for best cell growth.
    • Do not Flame Thermanox coverslips.
    • Do not handle with rubber gloves or other rubber products. Most rubber is toxic to cells and the toxic agent is transferred to the Thermanox surface.
    • Not recommended for phase contrast microscopy or techniques involving fluorescent stains.

    Suggestions for use

    • Cut to shapes or sizes with sterile scissors.
    • To prevent scratches, handle only by the corner or edges, preferably with sterile forceps.
    • A pick-up tab may be formed by bending one corner to a right angle with sterile forceps
    • Resterilize, if necessary, with 70% Isopropyl alcohol or expose to ultraviolet light overnight.
    • 10.5x22mm coverslip may be used with Nunc #156758 flat sided tube.
    • 25mm round coverslip may be used with 35x10mm dishes or 6 well plates.
    • After embedding, you may peel Thermanox coverslips off the epoxy, leaving cells or other objects in the mounting material or you may section them with the mount.

    Note: Thermanox coverslips may float due to air bubbles or surface tension. Air bubbles may form when the coverslip is placed in the medium, or if the medium is below incubator temperature. Bubbles may form as the medium comes up to the temperature releasing dissolved gas.

    Coverslips may be pushed to the bottom of a dish or other container with sterile forceps, pipette or gentle agitation. In Leighton tubes, a gentle shake will cause the coverslip to settle to the bottom of the vessel.

    To prepare semi-permanent/permanent preparations for light microscopy

    1. Fix cells grown on Thermanox coverslips in methanol, acetone or other fixative.
    2. Stain.
    3. Dehydrate in acetone.
    4. Clean in Xylene.
    5. Cover with a mountant.
    6. Place clear glass coverslip on top.

    Examine the cells through the glass coverslip and mountant for improved resolution.

    To Prepare for Transmission Electron Microscopy

    1. Fix cells with routine fixative (Glutaraldehyde).
    2. Post fix with OsO4 . Rinse and prestain.
    3. Dehydrate in ethanol followed by propylene oxide.
    4. Infiltrate with Epon.
    5. Invert on previously polymerized Epon Blank, wipe back and polymerize
    6. Remove coverslip by inverting on a warm hotplate and peeling off or by touching coverslip to dry ice. The coverslip will peel off leaving the cells on the Epon block.

    References

    1. Buckley, C.E. Coverslips for Use in Tissue Culture. Laboratory Equipment Digest, May, 1976
    2. Deter, R.L. Quantitative Morphological Analysis of Early Mouse Embryogenesis In Vitro. Perfusion Culture System. Tissue Preparation: Sampling. Journal Embryol. Exp. Morph. 40:19-100. 1977
    3. Pauli, B.U., Anderson , S.N., Mernoli, V.A. and Kuettner K.A. Development in an In Vitro and In Vivo Epithelial Tumor Model for the Study of Invasion. Cancer Research 40: 4571-4580. December, 1980
    4. Werb, Z., Bainton, D.F., and Jones, P.A. Degradation of Connective Tissue Matrices by Macrophages. Journal of Experimental Medicine, 152: 1537-1553. December, 1980
    5. Swierenga, S.H.H., Whitfield, J.F., and Morris, H.P. The Reproduced Extracellular Calcium Requeirement for Proliferation by Neoplastic Hepatocytes. In Vitro. 14 No 527-535. 1978
    6. Zembala. M., Lemmel, E.M., Uracz, W. Activation of Human Monocytes for Nitroblue Tetrazzolium Reduction and the Suppression of Lymphocytes Response to Mitogens. Clin.Exp. Immunol. 41: 309-316: 1980

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