GreenView™ Plus is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This single stain gives high sensitivity detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. GreenView™ Plus is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner.

GreenView™ Plus DNA Gel Stain, 10,000X is a concentrated GreenView Plus solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000X solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels.

Gel staining with GreenView™ Plus is compatible with downstream applications such as gel extraction and cloning. GreenView Plus is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Special Features High sensitive Non-toxic Non-mutagenic Detection of DNA and RNA Low cost 10,000X in DMSO Applications DNA gel stains

GreenView™, GreenView™ Plus, GreenView™ Ultra, and RedView™是GeneCopoeia公司最新研发具有安全无毒，灵敏度高，稳定性好的EB替代品, 使用方法同EB，可用于前染法和后染法，条带整齐不弯曲。GreenView™, GreenView™ Plus, and GreenView™ Ultra可在紫外灯或蓝光仪下观察发绿色荧光；RedView™可在紫外灯下观察发红色荧光。

核酸电泳是核酸研究的重要手段之一，其中溴化乙啶（EB）因其低廉的价格是分子生物学中使用最早和最为广泛的核酸电泳染色试剂。但是EB是一种强诱变剂，具有高致癌性，其废弃液必须进行净化处理，而且EB的检测灵敏度有限以及背景信号较高，目前正逐渐被灵敏度高以及安全可靠的新型核酸荧光染料所取代。

产品特点

Figure 1. Gel stain results with GreenView™, GreenView™ Plus, and GreenView™ Ultra (Left), and comparison results with SYBR Safe™ and GelGreen™ (Right). Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with GreenView™, GreenView™ Plus, GreenView™ Ultra, SYBR Safe™ and GelGreen™, respectively. Results show GreenView™ has similar sensitivity with SYBR Safe™ and GelGreen™, GreenView™ Plus and GreenView™ Ultra have much better sensitivity.

Figure 2. Gel stain results with RedView™, and GelRed™. Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with RedView™, and GelRed™, respectively. Results show RedView™ has similar sensitivity with GelRed™.

安全性对比：

此外，GreenView™, GreenView™ Plus, and RedView™ 具有较低的细胞膜通透性，不易与细胞内DNA结合，安全性优于SYBR Safe。

Figure 3. Cell permeability tests with SYBR Safe, GreenView, GreenView Plus, and RedView. Hela cells were incubated at 37℃ with 1X SYBR Safe, GreenView, GreenView Plus, and RedView for 30 min. GreenView Plus and RedView could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.