- 稀释倍数:6x。
-
直接加入电泳样品,每5ul加入1ul染料,可使用1000次。
- 凝胶无需染色。
-
使用传统的紫外凝胶成像仪或是蓝光成像,荧光颜色为:绿色。
说明书:
SafeGreen Loading Dye PDF(请点击查看)
SafeGreen™和SafeRed™是GeneCopoeia最新推出的安全灵敏,操作简单的次世代核酸电泳染料,电泳前只需将染料与DNA混合,上样,即可获得条带清晰的染胶效果,整个过程无需前染和后染。
产品特点
- 即用型,操作简单
- 安全无毒
- 灵敏度高
- 成本低
SafeGreen™ 和 SafeRed™ Loading Dye 含 6 × DNA 上样缓冲液中及两类常用的示踪染料 (Xylene Cyanol FF 和 Bromophenol Blue),用以监测电泳期间琼脂糖凝胶条带(呈现紫色和蓝色两个条带)迁移情况。DNA条带可通过 LED 或紫外光直接观察,无需前染和后染步骤。
Figure 4. Gel staining results with SafeGreen™ and SafeRed™ premixed with DNA ladder. 1 kb DNA Ladder (Cat# D001) was mixed with SafeGreen™ or SafeRed™ at a 5:1 ratio, then loaded onto each well. Gels were directly imaged on a UV transilluminator.
SafeGreen™ 和 SafeRed™ 无法穿透细胞膜进入活细胞,从而降低了细胞毒性(Figure 5)。
Figure 5. Cell permeability tests with SYBR Safe™, SafeGreen™, and SafeRed™. Hela cells were incubated at 37℃ with 1X SYBR Safe™, SafeGreen™, and SafeRed™ for 30 min. SafeGreen™ and SafeRed™ could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe™, which rapidly entered cells and stained nuclei.
核酸荧光染料选购指南
灵敏度 (dsDNA) |
Sensitive |
Highly sensitive |
Ultra sensitive |
Sensitive |
安全性 |
√ |
√ |
√ |
√ |
Gel electrophoresis |
√ |
√ |
√ |
√ |
dsDNA |
√ |
√ |
√ |
√ |
ssDNA |
√ |
√ |
||
RNA |
√ |
√ |
||
前染法 |
√ |
√ |
√ |
|
后染法 |
√ |
|||
胶回收 |
√ |
√ |
√ |
|
激发光源 |
蓝光/紫外 |
蓝光/紫外 |
蓝光/紫外 |
紫外 |
滤光片 |
SYBR filter |
SYBR filter |
SYBR filter |
EB filter |
货号 |
产品名称(点击可查看) |
描述 |
规格 |
目录价格(¥) |
AB-N100 |
无毒、无突变的DNA凝胶染料 |
500 µl |
400 |
|
AB-N101 |
无毒、无突变的DNA凝胶染料 |
500 µl |
600 |
|
AB-N103 |
无毒、无突变的DNA凝胶染料 |
500 µl |
800 |
|
AB-N102 |
无毒、无突变的DNA凝胶染料 |
500 µl |
600 |
|
AB-D012 |
无毒、无突变的DNA凝胶染料 |
1 mL |
300 |
|
AB-D013 |
无毒、无突变的DNA凝胶染料 |
1 mL |
300 |
|
AB-D001 |
DNA ladder |
250 µl |
200 |
|
AB-D002 |
DNA ladder |
250 µl |
270 |
|
AB-D003 |
DNA ladder |
250 µl |
270 |
|
AB-D016 |
DNA 上样缓冲液 |
5 ml |
70 |