Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca2+. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-3, AM is supplied as 1 mg solid (C213), special packing 20×50 μg (C214), and 1 mg/ml solution in DMSO (C215).
Specifications
Label: | Fluo-3 | |
Ex/Em: | 488/525 | |
Detection Method: | Fluorescent | |
Solubility: | DMSO, DMF | |
Molecular Formula: | C51H50Cl2N2O23 | |
Molecular Weight: | 1129.85 | |
CAS Number: | 121714-22-5 | |
Storage Conditions: | -20 ℃, protect from light | |
Shipping Condition: | Room Temperature | |
Applications Calcium indicator |
说明书:Fluo-3, AM(点击查看)
参考文献
References:
Nat Immunol (2000) 1:402-412
duct usage:
J Neurosci (1993) 13:4229-4245oduct usage:
J Neurochem (1992) 59:2193-2200
钙离子探针产品选择指南:
钙离子探针
货号 |
产品(点击查看) |
激发/发射波长(nm) |
规格 |
价格(¥) |
C200 |
– |
100 mg |
400 |
|
C203 |
– |
25 mg |
950 |
|
C207 |
488/515 |
1 mg |
1200 |
|
C208 |
488/515 |
20×50 μg |
1500 |
|
C209 |
Fluo-2, AM, 1mg/ml in DMSO |
488/515 |
1 ml |
1300 |
C213 |
488/525 |
1 mg |
1200 |
|
C214 |
488/525 |
20×50 μg |
1500 |
|
C215 |
Fluo-3, AM, 1mg/ml in DMSO |
488/525 |
1 ml |
1300 |
C224 |
340, 380/505 |
1 mg |
1200 |
|
C226 |
Fura-2, AM, 1mg/ml in DMSO |
340, 380/505 |
1 ml |
1300 |
C228 |
346/405, 485 |
1 mg |
900 |
|
C230 |
Indo-1, AM, 1 mg/ml in DMSO |
346/405, 485 |
1 ml |
1000 |
C233 |
550/578 |
1 mg |
1200 |
|
C234 |
550/578 |
20×50 μg |
1500 |
|
C235 |
Rhod-2, AM, 1mg/ml in DMSO |
550/578 |
1 ml |
1300 |
C238 |
– |
1 mg |
1500 |
|
C239 |
– |
250 μg |
1800 |
活细胞内的钙离子浓度检测:活细胞内钙离子浓度的检测(点击查看)