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钙离子探针，Fluo-3, AM，可透过细胞膜，1mg/ml in DMSO，即用型

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货号 AB-215
品牌 GeneCopoeia ( 经销商 )
CAS号 121714-22-5
规格/包装 1ml
单位个
储存条件低温
现货状态一个工作日

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Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca²⁺. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-3, AM is supplied as 1 mg solid (C213), special packing 20×50 μg (C214), and 1 mg/ml solution in DMSO (C215).

Specifications

Label:	Fluo-3
Ex/Em:	488/525
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Molecular Formula:	C₅₁H₅₀Cl₂N₂O₂₃
Molecular Weight:	1129.85
CAS Number:	121714-22-5
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Calcium indicator

说明书：Fluo-3, AM（点击查看）
参考文献

References:

Regulating T helper cell immunity through antigen responsiveness and calcium entry.Regulating T helper cell immunity through antigen responsiveness and calcium entry.

Bikah G, Pogue-Caley RR, McHeyzer-Williams LJ, McHeyzer-Williams MG
Nat Immunol (2000) 1:402-412

duct usage:

Calcium signaling of glial cells along mammalian axons.Calcium signaling of glial cells along mammalian axons.

Kriegler S, Chiu SY
J Neurosci (1993) 13:4229-4245oduct usage:

Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.

Lustig HS, von Brauchitsch KL, Chan J, Greenberg DA
J Neurochem (1992) 59:2193-2200

钙离子探针产品选择指南：

钙离子探针

货号	产品（点击查看）	激发/发射波长(nm)	规格	价格（¥）
C200	BAPTA, free acid	–	100 mg	400
C203	BAPTA, AM ester	–	25 mg	950
C207	Fluo-2, AM	488/515	1 mg	1200
C208	Fluo-2, AM	488/515	20×50 μg	1500
C209	Fluo-2, AM, 1mg/ml in DMSO	488/515	1 ml	1300
C213	Fluo-3, AM	488/525	1 mg	1200
C214	Fluo-3, AM	488/525	20×50 μg	1500
C215	Fluo-3, AM, 1mg/ml in DMSO	488/525	1 ml	1300
C224	Fura-2, AM	340, 380/505	1 mg	1200
C226	Fura-2, AM, 1mg/ml in DMSO	340, 380/505	1 ml	1300
C228	Indo-1, AM	346/405, 485	1 mg	900
C230	Indo-1, AM, 1 mg/ml in DMSO	346/405, 485	1 ml	1000
C233	Rhod-2, AM	550/578	1 mg	1200
C234	Rhod-2, AM	550/578	20×50 μg	1500
C235	Rhod-2, AM , 1mg/ml in DMSO	550/578	1 ml	1300
C238	Coelenterazine	–	1 mg	1500
C239	Coelenterazine h	–	250 μg	1800

活细胞内的钙离子浓度检测：活细胞内钙离子浓度的检测（点击查看）