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Fluo-4, AM is an analog of Fluo-3, AM with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Fluo-4, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-4 becomes fluorescent in the presence of free Ca2+. Fluo-4, AM is widely used for determining GPCR function via cell-based high-throughput screening.This indicator is useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.
Fluo-4, AM is supplied as 0.5 mg solid (C219), special packing 10×50 μg (C220), and 1 mg/ml solution in DMSO (C221).
Specifications
| Label: | Fluo-4 |
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| Ex/Em: | 488/506 nm | |
| Detection Method: | Fluorescent | |
| Molecular Formula: | C51H50F2N2O23 | |
| Molecular Weight: | 1096.95 | |
| Molecular Weight: | – | |
| Storage Conditions: | -20°C, protect from light | |
| Storage Conditions: | Room Temperature | |
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Applications |
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钙离子探针选择指南
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货号（可点击查看） |
产品（可点击查看） |
应用 |
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钙离子螯合剂，通过显微注射进入细胞，可以控制细胞液的钙离子浓度。 |
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可穿透细胞膜的钙离子螯合剂，实现对细胞内钙离子的监控。 |
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可穿透细胞膜的钙离子探针, Kd: 230 nM, Ex/Em = 488/515 nm。 |
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可穿透细胞膜的钙离子探针, Kd: 390 nM, Ex/Em = 488/525 nm。 |
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可穿透细胞膜的钙离子探针，Kd: 345 nM；Fluo-4 的荧光强度比 Fluo-3 强一倍。 |
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可穿透细胞膜的钙离子指示剂, Kd: 145 nM, Ex = 340/380 nm, Em = 505 nm。 |
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可穿透细胞膜的钙离子指示剂, Kd: 230 nM, Ex = 346 nm, Em = 405/485 nm。 |
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可穿透细胞膜的钙离子指示剂, Kd: 570 nM, Ex/Em = 550/578 nm。 |
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生物发光探针，海肾荧光素酶（Rluc）作用底物，用于测量细胞内钙离子浓度监控报告基因，具有高灵敏度和很宽的线性范围。Coelenterazine h，为水溶性探针，低毒性，可以用于活体或活细胞成像。Ex/Em = 550/578 nm。 |
