iClick™ EU Andy Fluor™ 488细胞成像分析试剂盒提供了一种简便、可靠的用于检测细胞、组织中RNA转录位点。由疾病、外界环境遭破坏或药物处理引起新合成RNA或RNA水平改变，分析这些变化是研究毒理学的重要部分。在这些分析中，新合成的RNA可以通过简单的两步法完成检测。第一步，炔基修饰的核苷类似物，5-乙炔基-尿嘧啶核苷（EU）加入细胞或注射到动物体内，可以掺入到新合成的RNA中。这种小分子标记物能够有效的掺入合成的RNA，而不是DNA中。利用叠氮化合物和炔烃之间的“click"反应，包含有叠氮化合物的荧光染料可以检测到这种修饰过的RNA。这种分析方法可以与抗体染色等多种成像检测技术兼容使用。

产品特点

Figure 1. Detection of newly synthesized RNA in cell. A549 cells were treated with 100 µM EU for 2 hr, then detected with Andy Fluor™ 488 azide (green), cells were counterstained with DAPI (blue).

Figure 2. Detection of newly synthesized RNA in mouse tissue. A mouse was injected intraperitoneally with 50 mg EU per kilogram body weight, then sacrificed at 0, 12, and 24 hr. The mouse intestine tissue was detected with Andy Fluor™ 488 azide (green), and counterstained with DAPI (blue).