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Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2′-deoxyuridine-5′-triphosphate]) (cat. no. C403A) is supplied as a 1 mM solution in TE buffer. Biotin-11-dUTP is also available in lyophilized form (cat. no. C403B).
Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The number '11’ is the number of atoms in the linker between biotin and dUTP.
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Specifications:
Concentration: 1 mM solution in TE buffer
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Applications
Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension.
