说明书:Cy5-X-dUTP (说明书PDF点击查看)
Cy5-X-dUTP (cat. no. C421A) is supplied as a 1 mM solution in TE buffer. Cy5-X-dUTP is also available in lyophilized form (cat. no. C421B).
Cy5-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between Cy5 and dUTP can improve the incorporation efficiency.
Specifications:
Excitation/Emission (nm): 650/665 nm |
Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.