典型应用：防止二硫键的生成，保持分散的结构。

产品名：TCEP-HCl；盐酸.磷酸三氯乙酯；Tris (2-Carboxyethyl) phosphine Hydrochloride

Cas：51805-45-9

货号: TCEP1

规格：10g,25g

分子式：C9H15O6P • HCl

分子大小：286.65 g/mol

储存：4°C.

产品描述：

TCEP-HCl是一种无臭的还原剂，可在蛋白质电泳中应用。TCEP是水溶性的，在各种各样的溶液中能稳定存在，结合马来酰亚胺作为半胱氨酸残基的标记使用，阻止半胱氨酸形成二硫键。作为一种还原剂，TCEP具有许多其他可取的特点，包括：

•TCEP在空气中是稳定，抗空气氧化。

•TCEP是无味的，反应可以在无通风厨的环境中进行。

•TCEP是有效的，低浓度的TCEP即可起到减少二硫键效果。

•TCEP非挥发性并且不与蛋白质中的其他功能基团反应。

•TCEP可作为在终浓度为DTT代替50mm。

TCEP与马来酰亚胺或卤代乙酰基化合物反应前无需去除。

Tris (2-Carboxyethyl) phosphine Hydrochloride
Molecular Biology Grade
Protease Free
C₉H₁₆O₆PCl
MW: 286.65 g/mol
PubChem Chemical ID: 2734570
TCEP 50 g and 100 g ships as multiple 25 g units to qualify for a small quantity exception to hazardous fees.
TCEP·HCl is an odorless reducing agent suitable for protein applications. The compound has many desirable features some of which are as follows:
1. TCEP·HCl is stable in air.
2. TCEP·HCl is odorless so reactions can be carried out on the bench without the need for a fume hood.
3. TCEP·HCl is efficient and can be used to reduce disulfide bonds at low concentrations.
4. TCEP·HCl is selective for disulfides and does not react with other protein sidechains.
5. TCEP·HCl may be used as a substitute for DTT at a final concentration of 50 mM.