Developed by the nephrology experts at Exocell, the Human Nephrin ELISA kit has been newly updated to remove any cross reactivity to albumin. Nephrin is a transmembrane protein expressed in renal glomerular podocytes; studies in experimental animals and human diabetes suggest nephrinuria is a marker for glomerular filtration dysfunction or podocyte injury. As an indirect competitive ELISA, Nephrin H uses an HRP labeled antibody to detect minute levels of nephrin, primarily in urine. The Nephrin H ELISA contains 1 x 96 well plate, and accounting for standard wells, 80 samples or 40 samples in duplicate (recommended) can be analyzed using one kit. Overnight incubation allows users to prepare the assay on the first day and assess the data on the second day.