hHbA1c is prepared from lysed human blood cells by sequential ion-exchange chromatographic methods. Essentially free of HbA1a, HbA1b and HbA0a0, the purified protein is validated by HPLC methods and carbohydrate analysis, and is suitable as a reference material for hHbA1ctesting. The reported concentration is determined by Drabkins assay.

The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20oC.

Short term storage should be done at 4oC. Long term storage should be completed at -60oC. Repeated cycles of freezing and thawing should be avoided.