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这是一款基于阴离子树脂技术的质粒小提试剂盒,可以降低内毒素最低到1EU的水平,远低于转染级质粒的内毒素含量要求。相比较,其他品牌没有类似的小量提取试剂盒,所以更具有便利性。
提取菌液量,高拷贝质粒(1-5毫升),低拷贝质粒(3-10毫升)。柱子载量:20ug。
更多信息:Brochure-Plasmid-DNA-purification-guide-EN.pdf (mn-net.com)
提取菌液量,高拷贝质粒(1-5毫升),低拷贝质粒(3-10毫升)。柱子载量:20ug。
更多信息:Brochure-Plasmid-DNA-purification-guide-EN.pdf (mn-net.com)
1st generation anion exchanger for purification of plasmid DNA
Application | High and low copy plasmid DNA purification |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Mini prep |
Package unit | 20 Prep(s), 100 Prep(s) |
Handling | Gravity flow |
Lysate clarification | Folded filter |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | 1−5 mL (high copy), 3−10 mL (low copy) |
Vector size | < 300 kbp |
Typical yield | 3−20 µg |
Theoretical binding capacity | 20 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | 1−10 EU/µg DNA |
Isopropanol precipitation | Centrifugation, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
Preparation time | 60 min/4−6 preps |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 18−25 °C |
Shelf life (from production) | 39 Months |
Hazardous material | Yes |