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Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
Extensive PCR testing is performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma
Protein concentrations determined by Lowry method
Endotoxin units measured by Limulus Amoebocyte Lysate assay
Matrigel Matrix gel stability tested for a period of 14 days at 37°C
Biological activity determined for each lot using a neurite outgrowth assay.
Source
Engelbreth-Holm-Swarm Mouse Tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
Application
Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Quality
Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
Extensive PCR testing is performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma
Protein concentrations determined by Lowry method
Endotoxin units measured by Limulus Amoebocyte Lysate assay
Matrigel Matrix gel stability tested for a period of 14 days at 37°C
Biological activity determined for each lot using a neurite outgrowth assay.
Source
Engelbreth-Holm-Swarm Mouse Tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
Application
Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
