Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
Extensive PCR testing is performed to screen for a number of pathogens
Tested and found negative for bacteria, fungi, and mycoplasma
Protein concentrations determined by Lowry method
Endotoxin units measured by Limulus Amoebocyte Lysate assay
Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
Biological activity is determined for each lot using a neurite outgrowth assay.
Source

Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application

Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice