This monoclonal antibody to puromycin provides a non-radioactive method to measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo.

Highlights:

• Allows for the simple evaluation and quantification of translation directly using standard immunochemical methods
• Advantageous alternative to traditional pulse-chase methods, which rely on radioactive amino acid labeling
• Compatible with Western Blot and ELISA applications
• Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 3RH11

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine and cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, and allows for the evaluation/quantification of translation directly using standard immunochemical methods.

Product Type:	Antibody
Name:	Anti-Puromycin (3RH11)
Host:	Mouse
Isotype:	IgG1 kappa
Clonality:	Monoclonal
Clone Name:	3RH11
Specificity:	This antibody recognizes puromycin.
Immunogen:	puromycin hydrochloride
Format:	Liquid
Purification Method:	Protein G purified
Buffer:	PBS with 0.02% Proclin 300
Tested Applications:	Western blotting (1:1,000), ELISA and Immunofluorescence microscopy.
Storage:	+4C (short-term), -20C (long-term); Avoid repeated freeze/thaw cycles.
Shipped:	Cold packs