Insulin，Transferrin，Selenium Solution (ITS)，100X Insulin，Transferrin，Selenium-A Solution (ITS-A)，100X Insulin，Transferrin，Selenium-X Solution (ITS-X)，100X (For Research Use Only. Not for use in diagnostic procedures) Description Insulin-Transferrin-Selenium (ITS) Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle’s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low density attachment of many adherent cell types. Safety information Wear appropriate protective eyewear, clothing, and gloves. Caution: Human origin materials are non-reactive (donor level) for anti-HIV 1 & 2, anti-HCV, and HBsAg. Handle in accordance with established bio-safety practices. Use • Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium. • In general, it is necessary to add 2–4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation. • Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2°C to 8°C. Product Catalog no. Amount Storage Shelf life Insulin，Transferrin，Selenium Solution (ITS)，100X LV-ITS001 10ml 2-8℃ 12months Insulin，Transferrin，Selenium-A Solution (ITS-A)，100X LV-ITS001A Insulin，Transferrin，Selenium-X Solution (ITS-X)，100X LV-ITS001X Contents Components Concentration (g/L) ITS ITS-A ITS-X Insulin 1.00 1.00 1.00 Transferrin 0.55 0.55 0.55 Sodium Selenite 0.00067 0.00067 0.00067 Sodium Pyruvate ----- 11.00 ----- Ethanolamine ----- ----- 0.20 QC TEST The results showed that there was no significant difference in the morphology of the primary pig hepatocytes, and the hepatocytes cultured by LV ITS formed a more prominent structure of bile duct. Order Tel:86-0755-28284050 Technical support: 86-19902901483