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anti-NLRP3/NALP3, mAb (Cryo-2)

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https://adipogen.com/ag-20b-0014-anti-nlrp3-nalp3-mab-cryo-2.html

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Product Details
Synonyms	NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein
Product Type	Monoclonal Antibody
Properties
Clone	Cryo-2
Isotype	Mouse IgG2b
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Application	Western Blot (see online protocol): (1μg/ml) Immunocytochemistry: (see J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012)) Immunoprecipitation: (in RIPA buffer, see Mol. Cell 49, 331 (2013)) Immunohistochemistry: (1:200, for a protocol see Nature 493, 67 (2012)) ChIP assay: 2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859 (2015))
Crossreactivity	Human Mouse
Specificity	Recognizes mouse and human NLRP3/NALP3.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control	Mouse IgG2b Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.