Intended Use: Mouse Cystatin C ELISA is designed to measure mouse cystatin c in a competitive format in urine, serum, saliva, or cell culture supernatant. It is for research purposes, and is not intended for diagnostic use.

Description: Mouse Cystatin C ELISA uses recombinant mouse cystatin C (Ala21-Ala140) as standard and a polyclonal antibody raised against recombinant cystatin C. This antibody does not react with mouse albumin or mouse IgG either in this ELISA or on Western Blot. Natural cystatin C showed dose-response curves that were parallel to the standard curves obtained using the Ethos Biosciences kit standards, indicating that this kit can be used to determine relative levels of native cystatin C. The assay is conducted in an indirect competitive mode and the results are reported as ng/ml mouse cystatin C.

The Mouse Cystatin C ELISA plates are coated with cystatin C and are supplied blocked and dried. The plates must be washed 2 times before use with wash buffer. To complete the assay, the diluted standards and samples are added to respective wells. The anti-cystatin C antibody is then added. This antibody interacts and binds with the mouse cystatin C in solution or with the cystatin C immobilized to the stationary phase, hence the notion of competitive binding. A subsequent reaction with HRP-conjugated second antibody then binds to the anti-cystatin C antibody bound to the stationary phase of the plate. The bound conjugated antibody is then detected using a chromogenic reaction. Color intensity in Mouse Cystatin C ELISA is inversely proportional to the logarithm of the concentration of mouse cystatin C in the fluid phase.