基本信息

概述

产品名称：β-Actin Rabbit Monoclonal Antibody
描述： β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-Actin are useful as loading controls for western blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
应用范围：WB, IHC
种属反应性：Human,Rat,Mouse,Zebrafish,Oryza sativa
免疫原：Full-length β-Actin protein of human

性能

形态：Liquid
存储条件：Store at -20°C or below. Avoid repeated freezing and thawing cycles.
存储溶液：1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
亚型：Mouse IgG
 

应用

应用说明：

WB: 1:5000~1:20000

IHC: 1:50~1:200

Optimal dilutions /concentrations should be determined by the end user

实验范例

 Western blot analysis of extracts of various cell lines with β-Actin rabbit mAb(6GX) diluted at 1:10000.

lane1: 293T，lane2: Hela，lane3: Mouse brain，lane4: Mouse testis，lane5: Mouse lung，lane6: Rat brain，lane7: Rat testis，lane8: Rat lung

Immunohistochemistry Staining of mouse liver tissue with β-Actin rabbit mAb(6GX) diluted at 1:100.