基本信息

概述

产品名称：GAPDH mouse monoclonal antibody
描述：Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
应用范围：WB
种属反应性：Human, Rat, Mouse
免疫原：Full-length GAPDH protein of human

性能

形态：Liquid
存储条件：Store at -20°C or below. Avoid repeated freezing and thawing cycles.
存储溶液：1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
亚型：Mouse IgG
 

应用

应用说明：

WB: 1:5000~1:20000

Optimal dilutions /concentrations should be determined by the end user

实验范例

 Western blot analysis of Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with GAPDH mouse mAb(1C4) diluted at 1:5000

Western blot analysis of Hela cell lysate with GAPDH mouse mAb(1C4) diluted at 1:5000(lane1) , 1:10000(lane2),1:20000(lane3) and 1:40000(lane4).