基本信息

概述

产品名称：β-Tubulin Mouse Monoclonal Antibody
描述：Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55 kDa. Antibodies against beta Tubulin are useful as loading controls for western blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
应用范围：WB, IF, IHC
种属反应性：Human, Rat, Mouse, Chicken, Fruit fly
免疫原：Full-length β-Tubulin protein of human

性能

形态：Liquid
存储条件：Store at -20°C or below. Avoid repeated freezing and thawing cycles.
存储溶液：1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
亚型：Mouse IgG
 

应用

应用说明：

WB: 1:5000~1:20000

IF: 1:100~1:1000

IHC: 1:100~1:1000

Optimal dilutions /concentrations should be determined by the end user

实验范例

 Western blot analysis of Hela cell lysate with β-Tubulin mouse mAb (3G7) diluted at 1:5000 (lane1), 1:10000 (lane2), 1:20000 (lane3) and 1:40000 (lane4).

Immunofluorescence Staining of SW480 cell with β-Tubulin mouse mAb(3G7). 

Fig.1: Nuclei stained with DAPI          Fig.2: ImmunoFluorescent analysis with β-Tubulin          Fig.3: Merge (1+2)

Immunohistochemistry Staining of human squamous cell carcinoma of cervical and prostate cancer with β-tubulin mouse mAb (3G7)