Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.

The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.

Spectral Properties
Absorbance: 450nm
Emission: 525nm

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/mol

Lyophilized

Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.