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Use CHEF* DNA size standards for size estimation of larger DNA fragments separated by pulsed field electrophoresis.
- Size range: 48.5–1,000 kb in 48.5 kb increments (λcl857Sam7 concatemers)
- Recommended gels: 0.8–1.0% or molecular biology grade agarose
Note: Cut a plug from a block into a size that will fit a well of your gel (5–8 plugs per block). The height of the plug should not extend above the well. After placing the plug into a well, seal the well with melted 1.0% low-melt agarose, and allow it to solidify. Store blocks at 4°C.
Pulsed Field Size Standards | Size Range |
0.2–2.2 Mb | |
4.9–98 kb | |
CHEF DNA Size Standard (1703635) | 48.5–1,000 kb |
1–3.1 Mb | |
8.3–48.5 kb |