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| CatalogNo: | A22054 |
| Brand: | ABclonal |
| ProductName: | Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Rabbit mAb |
| Gene Symbol: | Histone H3 |
| Alias: | H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A |
| GeneId: | 8290/8350 |
| Swiss-Prot: | Q16695/P68431 |
| Routing: | Synthetic peptide |
| Species: | Human |
| ProteinWeight: | 16kDa |
| ObservedMW: | 17kDa |
| Source: | Rabbit |
| Immunogen: | A synthetic acetylated peptide around K4 & K27 of human Histone (NP_003520.1). |
| AntigenSeq: | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY |
| CrossReactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Application: | ELISA, WB, IHC-P, IP, ChIP, ChIP-seq, CUT&Tag |
| RecommandDilution: | WB,1:2000 - 1:20000|IHC-P,1:50 - 1:200|IP,1:10000 - 1:60000|ChIP,1:50 - 1:200|ChIP-seq,1:20 - 1:100|CUT&Tag, 10⁵ cells /1 μg |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
| PositiveSamples: | HeLa TSA, NIH/3T3 TSA, C6 TSA |
| CelluarLocation: | Chromosome,Nucleus, |
| ResearchArea: | Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Immunology Inflammation,NF-kB Signaling Pathway |
| RRID: | |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
