Clone 7/4 (See other available formats) Regulatory Status RUO Other Names GKPIPNPLLGLDST epitope tag Isotype Mouse IgG2b, κ Antigen Details Structure 14 amino acid peptide (GKPIPNPLLGLDST) Function The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems. Biology Area Cell Biology Antigen References 1. Uhlen M, et al. 1990. Meth Enzymol. 185:129. 2. Hanke T, et al. 1995. J. Virol. Methods 53:149. 3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551. 4. Thomas SM, et al. 1988. Cell 54:891. Gene ID NA UniProt Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.