The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence.
宿主
Mouse
克隆类型
Monoclonal Antibody
亚型
IgG1
克隆号
2C5
稀释比例
WB 1:10000-15000. IP 1:100-300. IF 1:500-3000.
免疫原
A synthetic peptide coupled to KLH
纯化方法
Affinity purification
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存
Store at -20°C. Avoid freeze / thaw cycles.
应用
WB IF IP
规格
50ul 100ul
单位
支
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
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