产品介绍:
Agencourt AMPure XP PCR纯化系统利用固相顺磁珠技术用于PCR扩增公司的高通量纯化。大于100b片段会被选择性的吸附到磁珠上。过量的引物,核苷酸,盐和酶可以通过简单的洗涤程序得以去除。所得纯化的PCR产物基本上不含污染物。配合磁力架使用,比试剂盒的方法方便,纯度更高,节省成本。
典型应用范围是:
• PCR
• Sequencing (Sanger and Next Generation) ,测序,第二代测序。
• Genotyping and SNP detection ,基因分型和SNP检测。
• Fragment Analysis ,碎片分析。
• Primer Walking
• Cloning ,克隆产物。
The Agencourt AMPure XP PCR Purification systems utilize Agencourt’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. Agencourt
AMPure XP utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a
simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Agencourt AMPure XP purified products can be used in the following applications:
• PCR
• Sequencing (Sanger and Next Generation)
• Genotyping and SNP detection
• Fragment Analysis
• Primer Walking
• Cloning
The purification procedure is highly amenable to a variety of automation platforms because it
utilizes magnetic separation and requires no centrifugation or vacuum filtration. More
information on automating Agencourt XP can be found at
http://www.agencourt.com/technical/reagent_information//
使用方法:
Process Overview
1. Add 1.8ul AMPure XP per 1.0ul of PCR product
2. Bind PCR products to paramagnetic beads
3. Separation of beads + PCR from contaminants
4. Wash beads + PCR product 2x with 70% Ethanol to remove contaminants
5. Elute purified PCR product from beads
6. Transfer to new plate
使用方法和说明书:AMPureXPProtocolPDF
应用案例:http://www.ebiotrade.com/newsf/2013-9/2013912113611701.htm
实物图片:
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