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供应日期更新，2021.11.29，目前为期货状态，12月份到货。
BD Matrigel™ Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types. These include neurons,Sertoli cells, chick lens,and vascular endothelial cells,and hepatocytes.BD Matrigel Matrix will influence gene expression in adult rat hepatocytes，as well as three dimensional culture in mouse and human, mammary epithelial cells. It will support in vivo peripheral nerve regeneration, can be used for metabolism and toxicology studies,, and is the basis for several types of tumor cell invasion assays. BD Matrigel Matrix provides the substrate necessary for the study of angiogenesis both in vitro and in vivo. BD Matrigel Matrix also supports in vivo propagation of human tumors in immunosupressed mice.
BD Matrigel™ Basement Membrane Matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins to include laminin (a major component), collagen IV, heparan sulfate proteoglycans, and entactin/nidogen. BD Matrigel Matrix also contains TGF-beta, epidermal growth factor, insulin-like growth factor, fibroblast growth factor, tissue plasminogen activator, and other growth factors which occur naturally in the EHS tumor.
Wide Selection of Basement Membrane Matrices:
BD Matrigel Matrix Growth Factor Reduced (GFR) is suited for applications where a more highly defined basement membrane preparation is desired. Available in standard GFR, High Concentration (HC), and Phenol Red-Free formats.
BD Matrigel Matrix High Concentration is suited for in vivo applications where a high protein concentration augments growth of tumors. The high protein concentration also allows the BD Matrigel Matrix Plug to maintain its integrity after subcutaneous injection into mice. Available in Standard, Growth Factor Reduced (GFR), and Phenol Red-Free formats.
BD Matrigel Matrix Phenol Red-Free is recommended for assays which require color detection (i.e. fluorescence).
BD Matrigel hESC-qualified Matrix has been qualified as mTeSR™1-compatible by StemCell Technologies, thus eliminating the need for time-consuming screening, in order to provide the reproducibility and consistency essential for your human embryonic stem (hES) cell research. The mTeSR1 formulation and BD Matrigel Matrix have been shown to be a successful combination for feeder-free maintenance of different WiCell™ hES cell lines for up to 20 passages (mTeSR1, StemCell Technologies Cat. No. 05850).
Quality Control:
Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process Tested and found negative for bacteria, fungi, and mycoplasma Protein concentrations are determined by Lowry method Endotoxin units are measured by Limulus Amoebocyte Lysate assay BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor • • • • • • •
Typical Applications:
Cell Growth and Differentiation BD Matrigel Matrix is especially suited for the culture of polarized cells, such as epithelial cells. It promotes the differentiation of many cells types, including hepatocytes, neurons, mammary epithelial, endothelial, and smooth muscle cells.
In Vivo Angiogenesis Studies BD Matrigel Matrix HC can be used to assess in vivo angiogenic activity of different compounds by subcutaneous injection into mice (BD Matrigel Plug Assay). The plugs are subsequently removed and analyzed for the formation of blood vessels.
Augmentation of Tumor Growth in Nude Mice BD Matrigel Matrix HC has been shown to promote successful transplantation of many human tumor cells, including prostatic, breast, small-cell lung, colon, adrenal carcinomas, melanomas, and lymphoblastic leukemia cells. Also, it has been found to increase tumor growth rates
Ordering Information
更多产品介绍：
http://bd.com/resource.aspx?IDX=17841
http://catalog2.corning.com/Lifesciences/en-US/Shopping/ProductDetails.aspx?productid=354277(Lifesciences)&categoryname=
Corning® ，BD,Matrigel® hESC-Qualified Matrix, *LDEV-Free, 5mL (Product #354277)
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay.
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
Click here for Corning Matrigel Lot Selection and Reservation.
BD Matrigel™ Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types. These include neurons,Sertoli cells, chick lens,and vascular endothelial cells,and hepatocytes.BD Matrigel Matrix will influence gene expression in adult rat hepatocytes，as well as three dimensional culture in mouse and human, mammary epithelial cells. It will support in vivo peripheral nerve regeneration, can be used for metabolism and toxicology studies,, and is the basis for several types of tumor cell invasion assays. BD Matrigel Matrix provides the substrate necessary for the study of angiogenesis both in vitro and in vivo. BD Matrigel Matrix also supports in vivo propagation of human tumors in immunosupressed mice.
BD Matrigel™ Basement Membrane Matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins to include laminin (a major component), collagen IV, heparan sulfate proteoglycans, and entactin/nidogen. BD Matrigel Matrix also contains TGF-beta, epidermal growth factor, insulin-like growth factor, fibroblast growth factor, tissue plasminogen activator, and other growth factors which occur naturally in the EHS tumor.
Wide Selection of Basement Membrane Matrices:
BD Matrigel Matrix Growth Factor Reduced (GFR) is suited for applications where a more highly defined basement membrane preparation is desired. Available in standard GFR, High Concentration (HC), and Phenol Red-Free formats.
BD Matrigel Matrix High Concentration is suited for in vivo applications where a high protein concentration augments growth of tumors. The high protein concentration also allows the BD Matrigel Matrix Plug to maintain its integrity after subcutaneous injection into mice. Available in Standard, Growth Factor Reduced (GFR), and Phenol Red-Free formats.
BD Matrigel Matrix Phenol Red-Free is recommended for assays which require color detection (i.e. fluorescence).
BD Matrigel hESC-qualified Matrix has been qualified as mTeSR™1-compatible by StemCell Technologies, thus eliminating the need for time-consuming screening, in order to provide the reproducibility and consistency essential for your human embryonic stem (hES) cell research. The mTeSR1 formulation and BD Matrigel Matrix have been shown to be a successful combination for feeder-free maintenance of different WiCell™ hES cell lines for up to 20 passages (mTeSR1, StemCell Technologies Cat. No. 05850).
Quality Control:
Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process Tested and found negative for bacteria, fungi, and mycoplasma Protein concentrations are determined by Lowry method Endotoxin units are measured by Limulus Amoebocyte Lysate assay BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor • • • • • • •
Typical Applications:
Cell Growth and Differentiation BD Matrigel Matrix is especially suited for the culture of polarized cells, such as epithelial cells. It promotes the differentiation of many cells types, including hepatocytes, neurons, mammary epithelial, endothelial, and smooth muscle cells.
In Vivo Angiogenesis Studies BD Matrigel Matrix HC can be used to assess in vivo angiogenic activity of different compounds by subcutaneous injection into mice (BD Matrigel Plug Assay). The plugs are subsequently removed and analyzed for the formation of blood vessels.
Augmentation of Tumor Growth in Nude Mice BD Matrigel Matrix HC has been shown to promote successful transplantation of many human tumor cells, including prostatic, breast, small-cell lung, colon, adrenal carcinomas, melanomas, and lymphoblastic leukemia cells. Also, it has been found to increase tumor growth rates
Ordering Information
更多产品介绍：
http://bd.com/resource.aspx?IDX=17841
http://catalog2.corning.com/Lifesciences/en-US/Shopping/ProductDetails.aspx?productid=354277(Lifesciences)&categoryname=