Catalog：PMK041M                  PMK041S

Quantity：  50ul                           100ul

Applications：WB,IHC

Species Cross-Reactivity：H,R,M

Molecular Weight：29KD

Isotype：IgG1

Storage Buffer & Condition: 

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. 

Store at -20°C. Do not aliquot the antibody.

Recommended dilutions: 

WB: 1:5,000    IHC: 1:200

Specificity: 

Antibody can detects endogenous PCNA protein.

Background: 

PCNA is a marker for cells in early G1 phase and Sphase of the cell cycle. It is found in the nucleus 

and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the 

processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA 

is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants 

encoding the same protein have been found for PCNA. Pseudogenes of this gene have been

described on chromosome 4 and on the X chromosome.

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Application:

WB-Western blot

IHC-Immunochemistry

IF-Immunofluorescence

IP-Immunoprecipitation

ChIP-Chormatin Immunoprecipitation

Reactivity:

H-Human      R-Rat               M-Mouse      Mk-Monkey    Dg-Dog

Ch-Chicken   Hm-Hamster   Rb-Rabbit      Sh-Sheep        Pg-Pig