Anti-Flag monoclonal antibody is a widely used research reagent that targets the Flag peptide tag. The Flag peptide is a short amino acid sequence (DYKDDDDK) that can be fused to the N- or C-terminus of a protein of interest during recombinant protein expression. This tag allows for easy detection, purification, and analysis of the tagged protein. Key Applications of Anti-Flag Monoclonal Antibody: Protein Detection: Western blotting: Used to detect and quantify the expression of tagged proteins. Immunoprecipitation: To isolate tagged proteins from complex mixtures. Immunohistochemistry: For visualizing tagged proteins in cells and tissues. Flow cytometry: To analyze the expression of tagged proteins on cell surfaces. Protein Purification: Affinity chromatography: The Flag tag can be used to purify tagged proteins using anti-Flag affinity columns. Protein Localization: Immunofluorescence microscopy to visualize the subcellular localization of tagged proteins. Advantages of Using Anti-Flag Monoclonal Antibody: High specificity: The antibody binds with high affinity and specificity to the Flag peptide. Versatility: Can be used in various applications, making it a versatile tool for research. Ease of use: The Flag tag is relatively small and does not interfere with the function of most proteins. Wide availability: Anti-Flag monoclonal antibodies are readily available from multiple commercial sources.