Anti-V5 monoclonal antibody is another widely used research reagent that targets the V5 peptide tag. The V5 tag is a short amino acid sequence (GKPIPNPLLGLDST) derived from the p53 tumor suppressor protein. Like other tags, the V5 tag can be fused to the N- or C-terminus of a protein of interest to facilitate its detection, purification, and analysis. Key Applications of Anti-V5 Monoclonal Antibody: Protein Detection: Western blotting: Used to detect and quantify the expression of tagged proteins. Immunoprecipitation: To isolate tagged proteins from complex mixtures. Immunohistochemistry: For visualizing tagged proteins in cells and tissues. Flow cytometry: To analyze the expression of tagged proteins on cell surfaces. Protein Purification: Affinity chromatography: The V5 tag can be used to purify tagged proteins using anti-V7 affinity columns. Protein Localization: Immunofluorescence microscopy to visualize the subcellular localization of tagged proteins.