Anti-GST Monoclonal Antibody: A Powerful Tool for Protein Research Anti-GST monoclonal antibody is another widely used research reagent that targets the GST (glutathione S-transferase) tag. The GST tag is a relatively large protein (approximately 29 kDa) that is commonly fused to the N- or C-terminus of a protein of interest during recombinant protein expression. Key Applications of Anti-GST Monoclonal Antibody: Protein Detection: Western blotting: Used to detect and quantify the expression of tagged proteins. Immunoprecipitation: To isolate tagged proteins from complex mixtures. Immunohistochemistry: For visualizing tagged proteins in cells and tissues. Flow cytometry: To analyze the expression of tagged proteins on cell surfaces. Protein Purification: Affinity chromatography: The GST tag can be used to purify tagged proteins using glutathione-Sepharose beads. This is a very efficient and widely used method for protein purification. Protein Localization: Immunofluorescence microscopy to visualize the subcellular localization of tagged proteins.