Anti-His tag monoclonal antibody is another widely used research reagent that targets the His tag. The His tag is a short amino acid sequence (typically 10 consecutive histidine residues) that is often fused to the N- or C-terminus of a protein of interest during recombinant protein expression. Key Applications of Anti-His Tag Monoclonal Antibody: Protein Detection: Western blotting: Used to detect and quantify the expression of tagged proteins. Immunoprecipitation: To isolate tagged proteins from complex mixtures. Immunohistochemistry: For visualizing tagged proteins in cells and tissues. Flow cytometry: To analyze the expression of tagged proteins on cell surfaces. Protein Purification: Affinity chromatography: The His tag can be used to purify tagged proteins using nickel-nitrilotriacetic acid (Ni-NTA) resin. This is a highly efficient and widely used method for protein purification. Protein Localization: Immunofluorescence microscopy to visualize the subcellular localization of tagged proteins.