Anti-β-actin monoclonal antibody is a widely used research reagent that targets β-actin, a ubiquitous protein found in eukaryotic cells. β-actin is a component of the actin cytoskeleton, which plays a crucial role in cell structure, motility, and signaling. Key Applications of Anti-β-actin Monoclonal Antibody: Loading control: In Western blotting and other quantitative assays, anti-β-actin antibody is often used as a loading control to ensure equal protein loading between samples. This is important for accurate comparison of protein expression levels. Actin cytoskeleton studies: Anti-β-actin antibody can be used to study the organization and dynamics of the actin cytoskeleton. Advantages of Using Anti-β-actin Monoclonal Antibody: Ubiquitous expression: β-actin is expressed at high levels in most cell types, making it a reliable loading control. Consistent expression: β-actin expression levels are generally stable under various experimental conditions. High specificity: The antibody binds with high affinity and specificity to β-actin. Wide availability: Anti-β-actin monoclonal antibodies are readily available from multiple commercial sources.