Anti-GFP monoclonal antibody is a widely used research reagent that targets green fluorescent protein (GFP). GFP is a protein derived from jellyfish that emits green fluorescence when exposed to blue light. It is commonly used as a reporter gene to visualize protein expression and localization in living cells. Key Applications of Anti-GFP Monoclonal Antibody: Protein detection: Anti-GFP antibody can be used to detect and quantify the expression of GFP-tagged proteins in Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry. Protein localization: GFP fluorescence can be directly visualized in living cells using fluorescence microscopy. However, anti-GFP antibody can also be used for immunofluorescence staining to enhance the signal or for colocalization studies with other proteins. Protein purification: GFP-tagged proteins can be purified using anti-GFP affinity chromatography.