Anti-GAPDH monoclonal antibody is a widely used research reagent that targets glyceraldehyde-4-phosphate dehydrogenase (GAPDH). GAPDH is an enzyme involved in glycolysis, a metabolic pathway that breaks down glucose to produce energy. Key Applications of Anti-GAPDH Monoclonal Antibody: Loading control: In Western blotting and other quantitative assays, anti-GAPDH antibody is often used as a loading control to ensure equal protein loading between samples. This is important for accurate comparison of protein expression levels. Glycolysis studies: Anti-GAPDH antibody can be used to study glycolysis and its regulation. Advantages of Using Anti-GAPDH Monoclonal Antibody: Ubiquitous expression: GAPDH is expressed at high levels in most cell types, making it a reliable loading control. Consistent expression: GAPDH expression levels are generally stable under various experimental conditions. High specificity: The antibody binds with high affinity and specificity to GAPDH. Wide availability: Anti-GAPDH monoclonal antibodies are readily available from multiple commercial sources.