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Anti-V5-HRP is a monoclonal antibody that has been conjugated with horseradish peroxidase (HRP). HRP is an enzyme that catalyzes a reaction that produces a chemiluminescent signal. This conjugation allows for sensitive and specific detection of V5-tagged proteins in various applications.
Key Applications of Anti-V5-HRP:
Western blotting: Used to detect and quantify the expression of V5-tagged proteins.
Immunohistochemistry: For visualizing V5-tagged proteins in cells and tissues.
ELISA: To measure the concentration of V5-tagged proteins in biological samples.
Advantages of Using Anti-V5-HRP:
Enhanced sensitivity: HRP-conjugated antibodies provide amplified signal detection, allowing for the detection of low levels of target protein.
Versatility: Can be used in various applications, making it a versatile tool for research.
Wide availability: Anti-V5-HRP antibodies are readily available from multiple commercial sources.