Anti-Myc affinity gel is a chromatographic resin that contains immobilized anti-Myc monoclonal antibodies. This gel is used to purify proteins that have been fused to the Myc peptide tag. The Myc tag is a short amino acid sequence (EQKLISEEDL) that can be fused to the N- or C-terminus of a protein of interest during recombinant protein expression. How Anti-Myc Affinity Gel Works: Protein binding: The Myc-tagged protein binds specifically to the anti-Myc antibodies immobilized on the gel. Washing: Unbound proteins are washed away. Elution: The bound Myc-tagged protein is eluted from the gel using a competitive elution buffer that contains free Myc peptide. Advantages of Using Anti-Myc Affinity Gel: High specificity: The anti-Myc antibodies bind with high affinity and specificity to the Myc peptide, ensuring efficient protein purification. Versatility: Can be used to purify a wide range of Myc-tagged proteins. High yield: Can achieve high yields of purified protein. Ease of use: The purification process is relatively simple and can be automated.